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Dianova公司為德國(guó)的病理免疫組化產(chǎn)品供應(yīng)商，具備的抗體制備工藝及嚴(yán)格的質(zhì)控系統(tǒng)，產(chǎn)品在上享有良好的聲譽(yù)。

克隆號(hào)為H09的IDH1（isocitrate dehydrogenase 1, 異檸檬酸鹽脫氫酶1）鼠單克隆抗體為Dianova公司近期推出的產(chǎn)品之一，此抗體可特異性的檢測(cè)腦組織腫瘤中IDH1基因的R132H點(diǎn)突變。IDH1基因的第132密碼子的雜合突變是II 和III級(jí)神經(jīng)膠質(zhì)瘤（根據(jù)WHO的腫瘤學(xué)分類）的頻發(fā)事件。在大約70％的星形膠質(zhì)細(xì)胞瘤和少突神經(jīng)膠質(zhì)細(xì)胞瘤可發(fā)現(xiàn)IDH1基因的R132H點(diǎn)突變。

IDH1基因R132H突變?cè)诓糠帜X腫瘤中的頻發(fā)現(xiàn)象使克隆號(hào)為H09的IDH1抗體可高靈敏性和高特異性的用于此類腫瘤的鑒別診斷中：如從原發(fā)性膠質(zhì)母細(xì)胞瘤中區(qū)分出間變性星形細(xì)胞瘤、從毛細(xì)胞型星形細(xì)胞瘤或室管膜（細(xì)胞）瘤中區(qū)分出彌漫性星形細(xì)胞瘤（WHO分類II級(jí)），也可以用于具有IDH1突變的腫瘤細(xì)胞浸潤(rùn)前沿的與反應(yīng)性神經(jīng)膠質(zhì)增多癥的區(qū)分。因此，由于其高靈敏性和高特異性，此抗體在腦部腫瘤的分類分級(jí)中以及在檢測(cè)單一或少量浸潤(rùn)腫瘤細(xì)胞時(shí)是非常有用的。

DIA-404 – Anti-CD4 (Ms) from Rat (Clone: GHH4) for mouse FFPE tissue – 200 µl

Art.-Nr.                            DIA-404

Specificity                         CD4 (T4, MCH II co-receptor, T-helper cells)

Species Reactivity                  Mouse

Host Species                         Rat

Isotype                              IgG2b

Clone                                 GHH4

Clonality (Mono-/Polyclonal)          monoclonal

Application                       Immunohistochemistry (Paraffin-embedded Sections)

Conjugation                           unconjugated

Format                                lyophilisate

                                      antibody purified (from culture supernatant)

Product line / Topic                   CD markers

                                       immunohistochemistry

Intended Use                            for Research Use Only

Search Code                             DIA404

Manufacturer / Brand                    dianova

Reactivity

Antibody clone GHH4 has been developed and validated for the detection of murine CD4 in formalin-fixed paraffin-embedded tissue sections (mouse FFPE). Clone GHH4 identifies T helper cells in murine FFPE tissue by IHC.
CD4 is a marker for T helper cells. Besides this subpopulation of mature T cells (helper/inducer), CD4 is also expressed on most thymocytes and weakly on macrophages and dendritic cells.
Antigen recognition and subsequent T-cell activation is controlled by the interaction between TCR (T-cell receptor) and pMHC (peptide-major histocompatibility complexe). T-cell activation is often enhanced by a close cooperation of the TCR with two different co-receptors: CD8 and CD4. CD4 is a nonpolymorphous 55 kD glycoprotein belonging to the immunoglobulin superfamily. It acts as a co-receptor with the TCR during antigen-induced T cell activation and thymic differentiation by binding MHC class II and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail.
In humans, CD4 is expressed in the majority of T-cell lymphomas. CD4 plays an important role in the classification of lymphocytes in inflammatory lesions and malignant lymphomas. CD4 serves as HIV receptor on T cells, macrophages and brain and is downregulated by HIV proteins during AIDS progression.

IHC for CD4 in mouse FFPE tissue

Mouse spleen: The monoclonal antibody clone GHH4 specifically stains CD4 in standard FFPE mouse spleen. Incubation with antibody clone GHH4 (10μg/ml, corresponding to a 1:100 dilution) for 1h at RT. Detection with ImmPRESS HRP anti-Rat from Vector Laboratories (Picture courtesy of Chris Bonnas, Synaptic Systems, Göttingen, Germany).

General References:

1. Foti M et al. p56Lck anchors CD4 to distinct microdomains on microvilli. Proc Natl Acad Sci U S A. 2002, 99: 2008-13.
2. Brady et al. Crystal structure of domains 3 and 4 of rat CD4: relation to the NH2-terminal domains. Science 1993, 260:979-83
3. Janeway CA et al. The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation. Annu Rev Immunol. 1992, 10: 645-74.
4. Bierer BE et al. The biologic roles of CD2, CD4, and CD8 in T-cell activation. Annu Rev Immunol. 1989, 7: 579-99.
5. Fleischer B et al. Function of the CD4 and CD8 molecules on human T lymphocytes: regulation of T cell triggering. Jour-nal of Immunology 1996, 136: 1625-8
6. Wilde DB et al. Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lym-phocyte lines. J Immunol. 1983, 131: 2178-83.
7. Dialynas et al. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal anti-body GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983, 131: 2445-51.

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